About this Collection
Cell death and cell cycle assays are fundamental tools for investigating cellular responses to physiological and experimental stimuli. These assays enable researchers to characterize apoptosis, necrosis, cell cycle progression, and treatment-induced changes in cell fate. They are widely used in cancer biology, drug discovery, toxicology, immunology, and regenerative medicine to evaluate the mechanisms underlying cell survival, proliferation, and programmed cell death.
This collection provides standardized reference guides for commonly used cell death and cell cycle assays, describing their scientific principles, assay workflows, required reagents and instrumentation, experimental controls, data analysis, troubleshooting, advantages, limitations, and research applications to support accurate and reproducible assessment of cellular responses.
Cell Death & Cell Cycle Assays
Browse standardized reference guides for assays used to investigate apoptosis, necrosis, caspase activation, and cell cycle progression in cultured cells.
Annexin V or Propidium Iodide (PI) Apoptosis Assay
Differentiates viable, early apoptotic, late apoptotic, and necrotic cells based on phosphatidylserine exposure and membrane integrity.
Caspase-3/7 Activity Assay
Measures activation of executioner caspases as a quantitative indicator of apoptotic cell death.
Propidium Iodide (PI) Cell Cycle Analysis
Quantifies cellular DNA content by flow cytometry to determine cell cycle distribution and identify cell cycle arrest.