About this Collection
Cell function and behavior assays provide valuable insights into how cells respond to physiological and experimental conditions beyond simple measures of viability and proliferation. These assays evaluate processes such as cell migration, oxidative stress, cellular senescence, and functional responses that are critical in development, tissue repair, cancer biology, immunology, aging research, and drug discovery.
This collection provides standardized reference guides for commonly used cell function and behavior assays, describing their scientific principles, assay workflows, required reagents and instrumentation, experimental controls, data analysis, troubleshooting, advantages, limitations, and research applications to support accurate and reproducible functional characterization of cultured cells.
Cell Function & Behavior Assays
Browse standardized reference guides for assays used to investigate cell migration, oxidative stress, senescence, and other functional cellular responses.
Scratch (Wound Healing) Assay
Measures two-dimensional cell migration by monitoring closure of a defined scratch in a confluent cell monolayer.
Transwell Migration Assay
Evaluates directed cell migration through a porous membrane in response to chemotactic stimuli.
DCFDA Reactive Oxygen Species (ROS) Assay
Quantifies intracellular reactive oxygen species using a fluorogenic probe to assess oxidative stress.
Senescence-Associated β-Galactosidase (SA-β-Gal) Assay
Detects senescent cells through β-galactosidase activity as a hallmark of cellular aging and stress.