About this Collection
Cell proliferation assays are essential tools for measuring cell growth, division, and population expansion under normal and experimental conditions. They are widely used in cancer biology, developmental biology, regenerative medicine, drug discovery, toxicology, and tissue engineering to evaluate the effects of genes, growth factors, and therapeutic compounds on cellular proliferation.
This collection provides standardized reference guides for commonly used cell proliferation assays, describing their scientific principles, assay workflows, required reagents and instrumentation, experimental controls, data analysis, troubleshooting, advantages, limitations, and research applications to support accurate and reproducible assessment of cell proliferation.
Cell Proliferation Assays
Browse standardized reference guides for assays used to quantify cell growth, DNA synthesis, and cellular proliferation in cultured cells.
BrdU Incorporation Assay
Measures DNA synthesis by detecting bromodeoxyuridine incorporation into newly synthesized DNA during cell proliferation.
EdU Click Assay
Click chemistry-based assay for rapid and sensitive detection of DNA synthesis in proliferating cells.
Ki-67 Staining
Immunostaining method for identifying actively proliferating cells through expression of the Ki-67 nuclear protein.
PCNA Staining
Detects proliferating cell nuclear antigen (PCNA) to assess DNA replication and cell cycle progression.
Colony Formation (Clonogenic) Assay
Evaluates the long-term proliferative capacity and colony-forming ability of individual cells.