About this Collection
Cell viability and cytotoxicity assays are fundamental tools in cell biology, enabling researchers to evaluate cell health, metabolic activity, membrane integrity, and responses to experimental treatments. These assays play a central role in drug discovery, toxicology, cancer research, regenerative medicine, and basic biological studies by providing quantitative measures of cell survival, proliferation, and treatment-induced cytotoxicity.
This collection provides standardized reference guides for widely used cell viability and cytotoxicity assays, including their scientific principles, workflows, required reagents, data interpretation, experimental controls, troubleshooting, and common research applications to support reliable and reproducible laboratory experiments.
Cell Viability & Cytotoxicity Assays
Browse standardized reference guides for assays used to assess cell viability, metabolic activity, membrane integrity, and treatment-induced cytotoxicity in cultured cells.
MTT Assay
Colorimetric assay that measures cellular metabolic activity as an indicator of cell viability, proliferation, and cytotoxicity.
MTS Assay
Tetrazolium-based assay that generates a soluble formazan product for convenient measurement of viable cells.
WST-1 Assay
Water-soluble tetrazolium assay for rapid assessment of cell viability and proliferation through metabolic activity.
XTT Assay
Colorimetric viability assay that quantifies metabolically active cells by measuring formazan dye production.
Resazurin (Alamar Blue) Assay
Fluorometric and colorimetric assay for evaluating cell viability through the reduction of resazurin to resorufin.
CellTiter-Glo® Assay
Luminescent ATP-based assay that provides a rapid and sensitive measure of viable cell number.
Trypan Blue Exclusion Assay
Dye exclusion method for distinguishing viable and non-viable cells during routine cell counting.
LDH Release Assay
Measures lactate dehydrogenase released from damaged cells to evaluate membrane integrity and cytotoxicity.