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SOP-018: Operation of a Laboratory Centrifuge

Purpose​

To safely and consistently operate a laboratory centrifuge for routine mammalian cell culture applications while protecting samples, personnel, and equipment.

Scope​

This SOP applies to bench-top centrifuges used for processing mammalian cell suspensions, culture media, and laboratory reagents.

Responsibilities​

The operator is responsible for:

  • Inspecting the centrifuge before use.
  • Balancing all samples correctly.
  • Selecting the appropriate operating parameters.
  • Reporting equipment damage or malfunction.

Required Materials​

Reagents​

  • Cell suspension or laboratory sample

Equipment​

  • Laboratory centrifuge
  • Compatible rotor
  • Sterile centrifuge tubes
  • Tube rack
  • Personal protective equipment (PPE)

Procedure​

1. Pre-Use Inspection​

Before use, verify that:

  • The centrifuge is clean.
  • The rotor is securely installed.
  • Tubes are compatible with the selected rotor.
  • No visible damage is present.

2. Prepare Samples​

Ensure that:

  • Tubes are properly capped.
  • Samples are correctly labeled.
  • Tube volumes are appropriate for the selected rotor.

3. Balance the Rotor​

Arrange tubes so that opposing positions contain tubes of equal mass.

Do not operate the centrifuge with an unbalanced rotor.

4. Set Operating Parameters​

Select the required:

  • Speed
  • Time
  • Temperature (if applicable)

Verify the settings before starting the run.

5. Operate the Centrifuge​

Close the centrifuge lid securely.

Start the run.

Do not attempt to open the lid while the rotor is in motion.

Allow the rotor to stop completely before opening the centrifuge.

6. Remove Samples​

Carefully remove the tubes without disturbing pellets when present.

Inspect the tubes for leakage or damage before proceeding to the next step.

Acceptance Criteria​

ParameterAcceptance Criterion
Rotor balanceProperly balanced
TubesIntact with no leakage
EquipmentNormal operation without excessive vibration
SamplesSuccessfully processed

Critical Control Points​

  • Always balance the rotor before operation.
  • Use only compatible tubes and rotors.
  • Verify operating parameters before starting.
  • Do not exceed the manufacturer's recommended limits.
  • Wait until the rotor has completely stopped before opening the lid.

Documentation​

Record the following when required:

  • Date
  • Sample identification
  • Centrifuge identification
  • Operating parameters
  • Operator initials

Quality Control​

Verify:

  • Rotor is securely installed.
  • Samples remain properly labeled.
  • No tube leakage occurred.
  • No abnormal vibration or noise was observed.
  • Equipment is clean after use.

Troubleshooting​

ObservationPossible CauseCorrective Action
Excessive vibrationUnbalanced rotorStop the centrifuge immediately and rebalance the tubes
Loud or unusual noiseRotor improperly installed or damagedInspect the rotor and discontinue use if damage is suspected
Tube leakageDamaged or improperly sealed tubesReplace damaged tubes and repeat the procedure
Centrifuge lid will not openRotor has not completely stopped or safety lock engagedWait until the run has ended and follow the manufacturer's instructions

Safety Considerations​

  • Wear appropriate personal protective equipment (PPE).
  • Never operate the centrifuge with an unbalanced load.
  • Do not open the lid until the rotor has completely stopped.
  • Inspect tubes for cracks before use.
  • Clean spills immediately according to laboratory safety procedures.