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SOP-030: Response to Cell Culture Spills

Purpose​

To provide a standardized procedure for safely containing, disinfecting, and reporting spills involving mammalian cell cultures, culture media, and associated biological materials.

Scope​

This SOP applies to accidental spills occurring inside or outside a Class II Biosafety Cabinet during mammalian cell culture procedures.

Responsibilities​

The operator is responsible for:

  • Responding to spills promptly and safely.
  • Preventing the spread of contamination.
  • Using appropriate disinfectants.
  • Reporting significant spills or exposures according to institutional policy.

Required Materials​

Reagents​

  • Laboratory-approved disinfectant
  • 70% ethanol (for final surface cleaning, if applicable)

Equipment​

  • Absorbent laboratory wipes or paper towels
  • Biohazard waste container
  • Forceps or tongs (if required)
  • Personal protective equipment (PPE)

Procedure​

1. Secure the Area​

Stop work immediately.

Restrict unnecessary movement around the spill to prevent contamination.

If the spill occurs inside a biosafety cabinet, keep the cabinet operating throughout the cleanup.

2. Wear Appropriate PPE​

Before beginning cleanup, ensure appropriate PPE is worn.

Replace contaminated gloves if necessary.

3. Contain the Spill​

Cover the spill with absorbent material.

Avoid splashing or spreading contaminated