About this Collection
Molecular and imaging techniques are essential tools for investigating gene expression, protein abundance, cellular localization, and molecular mechanisms in biological research. These methods enable researchers to visualize cellular structures, detect specific biomolecules, and quantify changes in gene and protein expression across a wide range of experimental models.
This collection provides standardized reference guides for commonly used molecular and imaging techniques, describing their scientific principles, experimental workflows, required reagents and instrumentation, experimental controls, data analysis, troubleshooting, advantages, limitations, and research applications to support accurate and reproducible laboratory investigations.
Molecular & Imaging Techniques
Browse standardized reference guides for techniques used to visualize cellular structures, detect biomolecules, and analyze gene and protein expression.
Immunocytochemistry (ICC)
Detects and visualizes intracellular proteins using antigen-specific antibodies in fixed cultured cells.
Immunofluorescence (IF)
Uses fluorescently labeled antibodies to localize and visualize target proteins with high specificity.
Western Blot
Identifies and quantifies specific proteins following electrophoretic separation and antibody-based detection.
Quantitative Real-Time PCR (qPCR)
Measures gene expression by quantifying amplified nucleic acids in real time using fluorescent detection.