About this Collection
Cell culture supplements are added to basal media to enhance cell growth, viability, metabolism, and specialized cellular functions. These reagents provide essential nutrients, growth-promoting proteins, amino acids, buffering agents, and antimicrobial compounds that help create an optimal environment for maintaining healthy and reproducible cell cultures.
This collection provides standardized reference guides for commonly used cell culture supplements, including sera, serum replacements, amino acids, antibiotics, buffers, and chemically defined additives. Each guide describes the biological role, composition, preparation, storage, recommended use, and laboratory applications of individual supplements to support consistent and reproducible experimental workflows.
Browse Supplements
Explore standardized reference guides for serum replacements, amino acids, buffers, vitamins, lipids, and defined supplements used to support mammalian cell culture.
Albumax®
A lipid-rich bovine serum albumin supplement used as a chemically defined serum replacement for mammalian cell culture.
B-27™ Supplement
A serum-free supplement optimized for neuronal, neural stem cell, and organoid cultures.
Bovine Serum Albumin (BSA)
A multifunctional protein supplement used for stabilization, protein delivery, and serum-free culture applications.
Chemically Defined Lipid Concentrate
A defined lipid supplement that supports membrane synthesis, metabolism, and serum-free cell culture.
GlutaMAX™ Supplement
A stabilized L-glutamine substitute that improves media stability and supports long-term cell growth.
HEPES Buffer
A biological buffer that helps maintain physiological pH during cell handling outside a CO₂ incubator.
ITS Supplement
A chemically defined mixture of insulin, transferrin, and selenium used in serum-reduced and serum-free media.
KnockOut™ Serum Replacement
A defined serum replacement formulated to support embryonic and induced pluripotent stem cell cultures.
L-Glutamine
An essential amino acid supplement that supports cellular metabolism, protein synthesis, and proliferation.
MEM Vitamin Solution (100×)
A concentrated vitamin supplement used to enrich culture media and support cellular metabolism.
N-2 Supplement
A chemically defined supplement that supports serum-free culture of neurons and other specialized cell types.
Non-Essential Amino Acids (NEAA)
A balanced amino acid supplement that reduces the metabolic burden of amino acid synthesis in cultured cells.
β-Mercaptoethanol (2-Mercaptoethanol)
A reducing agent commonly added to stem cell and lymphocyte cultures to maintain a reduced intracellular environment.